In this guide we focus on explaining the ARC structure and its different components.UserNewbie ModeRead
Let's imagine a scenario where your project partner suggests at a conference to use this cool new Annotated Research Context (ARC) for your collaboration. Convinced by the versioning system and the single point of entry logic, you are motivated to set up your first own ARC after returning to the lab and fill it with your latest project results. Back home, however, you only remember the basic ARC structure and something about some isa.xlsx files. So how do you transfer your project into the empty ARC your project partner shared with you?
To answer this question, we will first take a look back at Viola's metadata example:
Viola investigates the effect of the plant circadian clock on sugar metabolism in W. mirabilis. For her PhD project, which is part of an EU-funded consortium in Prof. Beetroot's lab, she acquires seeds from a South-African Botanical Society. Viola grows the plants under different light regimes, harvests leaves from a two-day time series experiment, extracts polar metabolites as well as RNA and submits the samples to nearby core facilities for metabolomics and transcriptomics measurements, respectively. After a few weeks of iterative consultation with the facilities' heads as well as technicians and computational biologists involved, Viola receives back a wealth of raw and processed data. From the data she produces figures and wraps everything up to publish the results in the Journal of Wonderful Plant Sciences.
The entire information given in this example can be stored within an ARC. To illustrate the ARC specifications, we will highlight and explain every (sub)directory and ISA-file of the ARC with references to Viola's example.
The ISA investigation workbook allows you to record administrative metadata of your project. In Viola's example, the title of the project, the contact persons, and related publications correspond to such metadata. Besides that, the workbook can also contain a short description of your project, but also lists included studies with respective design types, assays, protocols, etc.. Although we recommend to use the ARC Commander for adding these metadata, you can of course fill the workbook (and also the isa.study.xlsx and isa.assay.xlsx) manually.
studies (sub)folders you can collect material and resources used within your studies. Corresponding information in Viola's project include the source of her seeds (South-African Botanical Society), how she grew the plants, and the design of the experiment (two-day time series, etc.).
In case your investigation contains more than one study, each of these studies is placed in an individual subdirectory. The "resources" directory allows you to store material samples or external data as virtual sample files. You can use the protocol subdirectory to store free-text protocols that describe how the samples or materials were created.
Every study contains one
isa.study.xlsx file to specify the characteristics of all material and resources. Resources described in a study file can be the input for one or multiple assays or workflows. The workbook contains (at least) two worksheets:
isa.investigation.xlsxusing the ARC Commander.
assays folder allows you to store data and metadata from experimental processes or analytical measurements. Each assay is a collection of files stored in a single directory, including corresponding metadata files in form of an
isa.assay.xlsx. Viola needs two subdirectories, one for her metabolomics and one for her transcriptomics dataset, respectively. Assay data files and free-text protocols are placed in individual subdirectories. Data files produced by an assay can be the input for one or multiple workflows.
Viola can annotate her experimental workflows of the metabolomics and transcriptomics assays with process parameters in the
isa.assay.xlsx file, which needs to be present for every assay. The workbook contains two or more worksheets, depending on the number of used protocols:
Note: Using the name of the protocol for the name of the worksheet can provide clarity.
isa.investigation.xlsxusing the ARC Commander.
In an ARC
workflows represent the processing steps used in computational analyses and other transformations of data originating from studies and assays. Typical examples include data cleaning and preprocessing, computational analysis, or visualization. The outcomes of these workflows ("run results") are stored in runs.
Viola received for her transcriptome and metabolome assays various processed data files, which she now can use to generate some nice plots. Additionally, the computational biologists sent her the code used for data processing, including an executable Common Workflow Language (CWL) file, which contains a standardized tool or workflow description. She stores these files in individual subdirectories for each workflow.
After Viola generated her plots, she placed them in individual subdirectories, specific to the run they were generated with. In general, you can use the runs folder to store plots, tables, or similar result files that derive from computations on study, assay, external data or other runs.
We hope that these examples nicely illustrated the ARC structure and that you are now ready to produce your own ARCs. Use the figure below as a cheat sheet to remember where to store which files. Or follow the ARC Commander QuickStart to try it out yourself.